Phosphate-Buffered Saline (PBS, 10X, pH 7.4)

COMPOSITION: 100 mM sodium phosphate dibasic, 20 mM potassium phosphate monobasic, 27 mM KCl and 1.37 M NaCl.

METHOD: Prepared in 18.2 megohms water and filtered through 1-micron filter.

PH: the pH of the 1X should be 7.4 ± 0.15.

NOTES: To make 1X, dilute 10-fold with DI water

STORAGE: Store at room temperature.