M9 Media Agar Plates

COMPOSITION: 1.28% Na2HPO4-7H2O, 0.30% KH2PO4, 0. 05% NaCl, 0.1% NH4Cl, 0.4 % Glucose, 2.0 mM Magnesium Sulfate, 0.1 mM Calcium Chloride, and 1. 5% Agar.
METHOD: All the ingredients were dissolved in deionized water, pH was adjusted to 7.0 ± 0.2 and autoclaved at 15 psi, 121 degree Celsius for 30 min on liquid cycle.
STORAGE: Store at 2-8 degree Celsius.

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