ChIP Dilution Buffer-II
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

ChIP Extraction Solution-1
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

ChIP Extraction Solution-2
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

ChIP High Salt Immune Complex Wash Buffer
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

ChIP IP Wash Buffer for Monoclonal Antibody
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

ChIP IP Wash Buffer for Polyclonal Antibody
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

ChIP LiCl Immunne Complex Wash Buffer-1
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

ChIP LiCl Immunne Complex Wash Buffer-2
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

ChIP Low Salt Immune Complex Wash Buffer
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

ChIP Lysis Buffer-III
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

ChIP Sonication Buffer
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

ChIP Swelling Buffer
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

ChIP Wash Buffer A
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

ChIP Wash Buffer B
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Chloramphenicol (10 mg/ml, EtOH)
Store at -20 degrees Celsius

Chlorophenol Red
Prepared in 18.2 megohms water and filtered through 1 micron filter.

Chloroquine (50 mM in PBS)
Prepared in low endotoxin PBS and filtered through 0.22-micron filter.

Chlortetracycline Hydrochloride (10 mg/ml, dH2O)
Prepared in 18.2 megohms low endotoxin water and filtered through 0.22-micron filter.

Citrate Buffer (0.2M, pH 4.0)
Prepared in 18.2 megohms low endotoxin water and filtered through 0.22 micron filter.

Citrate Buffer (0.2M, pH 5.0)
Prepared in 18.2 megohms low endotoxin water and filtered through 0.22 micron filter.

Citrate Buffer (0.2M, pH 6.0)
Prepared in 18.2 megohms low endotoxin water and filtered through 0.22 micron filter.

Citrate Buffer 0.5 M pH 2.5
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Citrate Buffer 0.5M, pH 3.0
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Citrate Buffer 0.5M, pH 3.5
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Citrate Buffer 0.5M, pH 4.0
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Citrate Buffer 0.5M, pH 4.5
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Citrate Buffered Saline or SSC 20X, pH 7.0
Prepared in 18.2 megohms water and filtered through 1 micron filter.

Citrate-Buffer 0.5 M pH 5.0
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Citrate-Buffer 0.5 M pH 5.5
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Citrate-Buffer 0.5 M pH 6.0
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Citrate-Buffer 0.5 M pH 7.0
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Citric Acid (0.2 N)
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Citric Acid (0.5N)
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Citric Acid (1 N)
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Clindamycin Hydrochloride (20 mg/ml, dH2O)
Prepared in 18.2 megohms low endotoxin water and filtered through 0.22-micron filter.

Cobalt Chloride (0.5 M)
Prepared in 18.2 megohms water and filtered through 0.22-micron filter.

Cold Lysis Buffer
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Colistin Sulfate (50 mg/ml, dH2O)
Prepared in 18.2 megohms low endotoxin water and filtered through 0.22-micron filter.

Congo Red Stain
Prepared in 18.2 megohms water and filtered through 1 micron filter.

Covaris Sonic Buffer(with SDS)
Prepared in 18.2 megohms water and filtered through 0.22- micron filter.

CP2D solution
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

CPD Solution
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

CPDA-1 Solution
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

CPDA-2 Solution
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Cresol Red
Prepared in 18.2 megohms water and filtered through 1 micron filter.

Crystal Violet Stain (Gram staining)
Prepared in 18.2 megohms water and filtered through 1 micron filter.

Crystal Violet Stain Solution
Prepared in 18.2 megohms water and filtered through 1 micron filter.

CTP
prepared in 18.2 megohms water, pH adjusted to 7.0 with NaOH, and filtered through 0.22 micron filter.

Cycloserine-D (20 mg/ml, dH2O)
Prepared in 18.2 megohms low endotoxin water and filtered through 0.22-micron filter.

Cytoplasmic Buffer
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Cytoplasmic Buffer
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Cytoplasmic Buffer-A
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Cytoplasmic Buffer-B
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

DABCO-Glycerol (Mounting Medium)
Prepared in 18.2 megohms water.

DABCO-PVA (Mounting Medium)
Prepared in 18.2 megohms water.

dATP
prepared in 18.2 megohms water, pH adjusted to 7.0 with NaOH, and filtered through 0.22 micron filter.

dCTP
prepared in 18.2 megohms water, pH adjusted to 7.0 with NaOH, and filtered through 0.22 micron filter.

Deionized Water
Deionized water prepared with Siemens water purification system on mixed bed resins.

Denaturing Lysis Buffer
Prepared in 18.2 megohms water and filtered through 1-micron filter.

Denaturing Solution
Prepared in 18.2 megohms water and filtered through 1-micron filter.

Denhardt Solution (100X)
Prepared in 18.2 megohms water and filtered through 1-micron filter.

Denhardt Solution (50X)
Prepared in 18.2 megohms water and filtered through 1-micron filter.

Destomycin (20 mg/ml, dH2O)
Prepared in 18.2 megohms low endotoxin water and filtered through 0.22-micron filter.

Dextrose Gelatin Veronal DGV
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

dGTP
prepared in 18.2 megohms water, pH adjusted to 7.0 with NaOH, and filtered through 0.22 micron filter.

Dialysis tubing wash buffer (5X)
Prepared in 18.2 megohms water and filtered through 1 micron filter.

Diluting solution (2X)
Prepared in 18.2 megohms water and filtered through 1 micron filter.

DIPSO Buffer 0.2M, pH 7.0
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

DIPSO Buffer 0.2M, pH 7.5
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

DIPSO Buffer 0.2M, pH 8.0
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

DIPSO Buffer 0.2M, pH 8.5
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Distilled Water
Double distilled and autoclaved-Sterile water.

DNA Binding Beads Mix
All the ingredients except Magnetic beads were dissolved in nuclease-free water and were autoclaved at 15 psi (121 0C) for 30 min. 0.1% Magnetic beads solution was prepared in Tris-NaCl-EDTA-PEG based buffer.

DNA Marker (High Range) 1 Kilo Base Pair Ladder
prepared in 18.2 megohms water. Water was autoclaved at 15 psi for 30 min on liquid cycle.

DNA Markers (Low Range) 250 Base Pair Ladder
prepared in 18.2 megohms water. Water was autoclaved at 15 psi for 30 min on liquid cycle.

DNase/RNase free water (non DEPC treated)
Distilled and steam sterilized at 121 degree C/15psi for 60 min.

dNTP Mix
Prepared in 18.2 megohms water, pH adjusted to 7.0 with NaOH, and filtered through 0.22 micron filter.

dNTP Set
Prepared in 18.2 megohms water, pH adjusted to 7.0 with NaOH, and filtered through 0.22 micron filter.

Doxorubicin Hydrochloride (10 mg/ml, dH2O or DMSO)
Prepared in either 18.2 megohms low endotoxin water and filtered through 0.22-micron filter or in DMSO.

Doxycyclin Hydrochloride (20 mg/ml dH2O)
Prepared in 18.2 megohms low endotoxin water and filtered through 0.22-micron filter.

dTTP
prepared in 18.2 megohms water, pH adjusted to 7.0 with NaOH, and filtered through 0.22 micron filter.

Dulbecco’s Phosphate Buffered Saline (no calcium, no magnesium)
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Dulbecco’s Phosphate Buffered Saline (With calcium, with magnesium. No Phenol red)
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Dulbecco’s Phosphate Buffered Saline 10X (no calcium, no magnesium)
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Dulbecco’s Phosphate Buffered Saline 10X (no calcium)
Both the solutions are prepared in 18.2 megohms low endotoxin water and filtered through 0.22 micron filter.

Dulbecco’s Phosphate Buffered Saline 10X (no magnesium )
Both the solutions are prepared in 18.2 megohms low endotoxin water and filtered through 0.22 micron filter.

dUTP
prepared in 18.2 megohms water, pH adjusted to 7.0 with NaOH, and filtered through 0.22 micron filter.

E. coli Lysis Buffer
Prepared in 18.2 megohms water and filtered through 1 micron filter.

E. coli Lysis Buffer-II
Prepared in 18.2 megohms water and filtered through 1 micron filter.

E. coli Sample Buffer
Prepared in 18.2 megohm water.

Earls Salt Solution (complete)
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Earls Salt Solution (no calcium, no magnesium)
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Earls Salt Solution (no calcium)
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Earls Salt Solution (no magnesium)
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Earls Salt Solution (no Phenol Red)
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Earls Salt Solution (no sodium bicarbonate)
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Earls Salt Solution 10X (no calcium, and no magnesium)
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Earls Salt Solution 10X (no calcium, no magnesium, and no phenol red)
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Earls Salt Solution 10X (no calcium)
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Earls Salt Solution 10X (no magnesium)
Both the solutions prepared in 18.2 megohms low endotoxin water and filtered through 0.22 micron filter.

Earls Salt Solution 10X (no sodium bicarbonate)
Both the solutions prepared in 18.2 megohms low endotoxin water and filtered through 0.22 micron filter.

Earls Salt Solution 10X (with Phenol Red)
Both the solutions are prepared in 18.2 megohms low endotoxin water and filtered through 0.22 micron filter.

EBC Lysis Buffer
Prepared in 18.2 megohms water and filtered through 1-micron filter.

EDTA (0.5 M, pH 7.4), autoclaved
Prepared in 18.2 megohms water, pH adjusted with potassium hydroxide and autoclaved at 15 psi for 1hr on liquid cycle.

EDTA (0.5M, pH 7.4), autoclaved
Prepared in 18.2 megohms water, pH adjusted with sodium hydroxide and autoclaved at 15 psi for 1hr on liquid cycle.

EDTA (0.5M, pH 8.0), autoclaved
Prepared in 18.2 megohms water, pH adjusted with potassium hydroxide and autoclaved at 15 psi for 1hr on liquid cycle.

EDTA (0.5M, pH 8.0), autoclaved
Prepared in 18.2 megohms water, pH adjusted with NaOH and autoclaved at 15 psi for 1hr on liquid cycle.

EDTA (14%), pH 7.4
prepared in 18.2 megohms water and autoclaved at 15 psi for 60 min on liquid cycle.

EDTA (17%), pH 7.0
prepared in 18.2 megohms water and autoclaved at 15 psi for 60 min on liquid cycle.

EGTA (0.5M, pH 7.4) autoclaved
Prepared in 18.2 megohms water and autoclaved at 15 psi for 30 min on liquid cycle.

EGTA (0.5M, pH 8.0), autoclaved
Prepared in 18.2 megohms water and autoclaved at 15 psi for 30 min on liquid cycle.

EGTA (1M, pH 7.4) autoclaved
Prepared in 18.2 megohms water and autoclaved at 15 psi for 30 min on liquid cycle.

EGTA (1M, pH 8.0) autoclaved
Prepared in 18.2 megohms water and autoclaved at 15 psi for 30 min on liquid cycle.

Elution buffer
Prepared in 18.2 megohms water and filtered through 0.22- micron filter.

Elution Buffer (His-tag protein purification from E.coli and insect cells)
Prepared in 18.2 megohms water and filtered through 1 micron filter.

Elution Buffer, pH 8.0 (His-tag protein purification from mammalian cells)
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Enterococcosel Agar Plates with vancomycin ( Vancomycin 20 micro gram/ml)
All the components for the Enterococcosel Agar Plates (except vancomycin) were mixed thoroughly in 1 L of purified water, followed by frequent agitation and boiling for 1 minute to completely dissolve the powder. Sterilized by autoclaving at 15 psi pressure (121 degree Celsius) for 15 minutes. After autoclaving, the media was cooled down to 50 to 55 degree Celsius. Vancomycin was added to Enterococcosel Agar and poured into 100 mm petri-dishes under sterile conditions.

Eosin B Stain Solution
Prepared in 18.2 megohms water and filtered through 1 micron filter.

Eosin Y Stain Solution
Prepared in 18.2 megohms water and filtered through 1 micron filter.

Ertapenem Sodium Salt (100 mg/ml, dH2O)
Prepared in 18.2 megohms low endotoxin water and filtered through 0.22-micron filter.

Erythromycin (50 mg/ml, EtOH)
Prepared in ethanol.

Erythrosin B Stain Solution
Prepared in 18.2 megohms water and filtered through 1 micron filter.

Factor Xa Protease Digestion Buffer (His-tag protein purification from mammalian cells)
Prepared in 18.2 megohms water and filtered through 1 micron filter.

Fast Green
Prepared in 18.2 megohms water and filtered through 1 micron filter.

FBS Stain Buffer
Prepared in 18.2 megohms-cm ┬▒ 1 low endotoxin water and filtered through 0.22-micron filter.

FCS Blocking buffer in TBS
Filtered through 1-micron filter.

Ferrous Sulfate (0.5 M)
Prepared in 18.2 megohms water and filtered through 0.22-micron filter.

Fluorescein
Prepared in 18.2 megohms water and filtered through 1 micron filter.

Formaldehyde (10%) in PBS
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Formaldehyde(4%) in PBS
Prepared in 18.2 megohms water.

Formic Acid (1N)
Prepared in 18.2 megohms water.

Formic Acid (2N)
Prepared in 18.2 megohms water.

Formic Acid (5N)
Prepared in 18.2 megohms water.

FSB
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Gelatin (10%).
Prepared in 18.2 megohms water.

Gelatin Veronal Buffer (GVB)
Prepared in 18.2 megohms water and filtered through 1 micron filter.

Gelatin Veronal Buffer (GVB)
Prepared in 18.2 megohms water and filtered through 1micron filter.

Gelatin Veronal Buffer with EDTA (GVBE)
Prepared in 18.2 megohms water and filtered through 1 micron filter.

Gelatin Veronal Buffer with EDTA (GVBE)
Prepared in 18.2 megohms water and filtered through 1 micron filter.

Gelatin Veronal Buffer with Mg & Ca (GVB++)
Prepared in 18.2 megohms water and filtered through 1micron filter.

Gelatin Veronal Buffer with Mg & Ca (GVB++)
Prepared in 18.2 megohms water and filtered through 1 micron filter.

Gelatin Veronal Buffer with Mg & EGTA (GVBMG)
Prepared in 18.2 megohms water and filtered through 1 micron filter.

Gelatin Veronal Buffer with Mg & EGTA (GVBMG)
Prepared in 18.2 megohms water and filtered through 1 micron filter.

Gelatin with 0.05% Tween in PBS
Prepared in 18.2 megohms water and filtered through 1 micron filter.

Gelatin with 0.05% Tween-20 in PBS
Prepared in 18.2 megohms water and filtered through 1 micron filter.

Gelatin with 0.05% Tween-20 in TBS
Prepared in 18.2 megohms water and filtered through 1-micron filter.

Gelatin with 0.05% Tween-20 in TBS
Prepared in 18.2 megohms water and filtered through 1-micron filter.

Gelatin- Blocking Buffer in PBS (with azide)
Prepared in 18.2 megohms water and filtered through 1-micron filter.

Gelatin-Blocking Buffer in PBS
Prepared in 18.2 megohms water.

Gelatin-Blocking Buffer in TBS
Prepared in 18.2 megohms water and filtered through 1-micron filter.

Gelatin-Blocking Buffer in TBS (with azide)
Prepared in 18.2 megohms water and filtered through 1-micron filter.

Geldanamycin (1 mg/ml, DMSO)
Prepared in DMSO.

General Molecular Biology Grade Water
Distilled and steam sterilized at 121 degree C/15psi for 60 min. Ideal for all general laboratory applications.

Genetcin-G-418 (50 mg/ml, dH2O)
Prepared in 18.2 megohms low endotoxin water and filtered through 0.22-micron filter.

Gentamicin (20 mg/ml, dH2O)
Prepared in 18.2 megohms low endotoxin water and filtered through 0.22-micron filter.

Gey’s Balanced Salt Solution (no calcium, no magnesium, and no phenol red)
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Gey’s Balanced Salt Solution (no magnesium)
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.

Gey’s Balanced Salt Solution (no phenol red)
Prepared in 18.2 megohms water and filtered through 0.22 micron filter.