DNA Binding Beads Mix
All the ingredients except Magnetic beads were dissolved in nuclease-free water and were autoclaved at 15 psi (121 0C) for 30 min. 0.1% Magnetic beads solution was prepared in Tris-NaCl-EDTA-PEG based buffer.
Enterococcosel Agar Plates with vancomycin ( Vancomycin 20 micro gram/ml)
All the components for the Enterococcosel Agar Plates (except vancomycin) were mixed thoroughly in 1 L of purified water, followed by frequent agitation and boiling for 1 minute to completely dissolve the powder. Sterilized by autoclaving at 15 psi pressure (121 degree Celsius) for 15 minutes. After autoclaving, the media was cooled down to 50 to 55 degree Celsius. Vancomycin was added to Enterococcosel Agar and poured into 100 mm petri-dishes under sterile conditions.