REAGENTS FOR YEAST RESEARCH


Yeast Media and Plates

Catalog # Product Name Pricing
LB-714 10% Glycerol (Sterile)
10% Glycerol prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 Psi, 121 degree Celsius for 60 min. Store at room temperature.
500ml $30.00
LB-751 20% Galactose
Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. Store at 2-8 degree Celsius.
250ml $40.00
LB-749 20% Glucose
Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. Store at 2-8 degree Celsius.
250ml $40.00
LB-715 20% Glycerol (Sterile)
20% glycerol prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 Psi, 121 degree Celsius for 60 min. Filtered through 0.22-micron filter. Store at 2-8 degree Celsius.
250ml $40.00
LB-757 20% Lactate
pH 6.0 with KOH. Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. Store at 2-8 degree Celsius.
250ml $40.00
LB-755 20% Potassium Acetate
Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. Store at 2-8 degree Celsius.
250ml $40.00
LB-753 20% Raffinose
Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. Store at 2-8 degree Celsius.
250ml $40.00
LB-725 AHC Medium
0.67% Yeast Nitrogen base, 1% Casein Hydrolysate acid, and 0.002% Adenine sulfate, pH 5.8 ± 0.15. All ingredients dissolved in 18.2 megohms-cm ± 1 water and autoclaved at 15 Psi, 121 degree Celsius for 30 min on liquid cycle. Store at room temperature and protect from direct light.
500ml $45.00
1L $60.00
LB-727 AHC Plates
0.67% Yeast Nitrogen base, 1% Casein Hydrolysate acid, 0.002% Adenine sulfate, 2% Agar, pH 5.8 ± 0.15. All ingredients dissolved in 18.2 megohms-cm ± 1 water and autoclaved at 15 Psi, 121 degree Celsius for 30 min on liquid cycle. Store at 2-8 degree Celsius and protect from direct light.
20 Plates $60.00
LB-759 Biotin (0.02%)
Prepared in 50 mM KOH. 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. Store at 2-8 degree Celsius.
100ml $40.00
LB-710 BMYG Media
1% Yeast extract, 2% Peptone in 100 mM Potassium phosphate pH 6.0 ± 0.15. All ingredients dissolved in 18.2 megohms-cm ± 1 water and autoclaved at 15 Psi, 121 degree Celsius for 60 min on liquid cycle. Store at 2-8 degree Celsius.
1L $35.00
2L $65.00
LB-712 BMYG Media (10X without Glucose)
Yeast Nitrogen base with Ammonium sulfate. 1.3% Yeast nitrogen base, 2% Peptone in 100 mM Ammonium sulfate pH 6.0 ± 0.15 containing vitamins and trace elements. All ingredients dissolved in 18.2 megohms-cm ± 1 water and autoclaved at 15 Psi, 121 degree Celsius for 60 min on liquid cycle. Store at 2-8 degree Celsius and protect from direct light.
500ml $65.00
LB-713 BMYG Media(10X) with Glucose
Yeast Nitrogen base with Ammonium sulfate. 1.3% Yeast nitrogen base, 2% Peptone, 5% Glucose in 100 mM Ammonium sulfate pH 6.0 ± 0.15 containing vitamins and trace elements. All ingredients dissolved in 18.2 megohms-cm ± 1 water and autoclaved at 15 Psi, 121 degree Celsius for 60 min on liquid cycle. Store at 2-8 degree Celsius and protect from direct light.
500ml $65.00
LB-747 CaS 2 Medium
1 M Sorbitol,10 mM Tris-HCl, pH 7.5, 10 mM CaCl2 and 40% PEG in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. Store at 2-8 degree Celsius.
250ml $40.00
LB-745 SCE 2 Medium
1 M Sorbitol, 10 mM Sodium citrate buffer and 1 mM EDTA pH 5.8. All ingredients dissolved in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. Store at 2-8 degree Celsius.
250ml $40.00
LB-743 SCE Medium
1M sorbitol, 0.1M sodium citrate and 60 mM EDTA, pH7.0. All ingredients dissolved in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. Store at 2-8 degree Celsius.
250ml $40.00
LB-729 SD (Synthetic Dextrose) Medium
2% Dextrose, 0.17% Yeast Nitrogen base, 0.5% Ammonium Sulfate. All ingredients dissolved in 18.2 megohms-cm ± 1 water and autoclaved at 15 Psi, 121 degree Celsius for 60 min. Store at 2-8 degree Celsius and protect from direct light.
500ml $25.00
1L $40.00
LB-731 SD-Agar Plates
2% Dextrose, 0.17% Yeast Nitrogen base, 0.5% Ammonium Sulfate and 1.5% Agar. All ingredients dissolved in 18.2 megohms-cm ± 1 water and autoclaved at 15 Psi, 121 degree Celsius for 60 min. Store at 2-8 degree Celsius and protect from direct light.
20 Plates $60.00
LB-741 SE 2 Medium
1 M Sorbitol, 25 mM EDTA, pH 8.0 dissolved in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. Store at 2-8 degree Celsius.
250ml $40.00
LB-734 Sorbitol-Agar Plates
1 M Sorbitol, 0.5% Ammonium sulfate, 0.17% Yeast Nitrogen base, 2% Glucose and 2% Agar, pH 5.8 ± 0.15. All ingredients dissolved in 18.2 megohms-cm ± 1 water and autoclaved at 15 Psi, 121 degree Celsius for 30 min on liquid cycle. Store at 2-8 degree Celsius.
20 Plates $60.00
LB-733 Sorbitol-Medium
1 M Sorbitol, 0.5% Ammonium sulfate, 0.17% Yeast Nitrogen base and 2% Glucose, pH 5.8± 0.15. All ingredients dissolved in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi, 121 degree Celsius for 30 min on liquid cycle. Store at 2-8 degree Celsius.
250ml $30.00
500ml $50.00
LB-739 SOS Medium
1 M Sorbitol, 0.3X YPD and 10 mM CaCl2. All the ingredients were dissolved in in 18.2 megohms-cm ± 1 in water, autoclaved at 15 psi, 121 degree Celsius for 30 min on liquid cycle. Store at 2-8 degree Celsius.
250ml $40.00
LB-737 Sporulation Agar Plates
1% potassium acetate, 0.1% yeast extract, 0.15% glucose, 1 M Sorbitol, 0.5% Ammonium sulfate, 0.17% Yeast Nitrogen base, 2% Glucose and 2% agar pH 5.8± 0.15. All ingredients dissolved in 18.2 megohms-cm ± 1 water and autoclaved at 15 Psi, 121 degree Celsius for 60 min on liquid cycle. Store at 2-8 degree Celsius.
20 Plates $60.00
LB-551 Super Broth Media
Tryptone 3,8%, Yeast Extract 2%, NaCl 0.5%, pH 7.0 +/- 0.1. All the components for the Super Broth Media were mixed thoroughly in 1 L of deionized water and sterilized by autoclaving at 15 psi pressure (121°C) for 30 minutes. Stored at RT.
1L $60.00
LB-718 YPD Media
1% Yeast extract, 2% Peptone and 2% Dextrose. All ingredients dissolved in 18.2 megohms-cm ± 1 water and autoclaved at 15 Psi, 121 degree Celsius for 30 min on liquid cycle. Store at room temperature and protected from direct light..
500ml $25.00
1L $40.00
LB-721 YPD Plates
1% Yeast extract, 2% Peptone, 2% Dextrose and 2% Agar. All ingredients dissolved in 18.2 megohms-cm ± 1 water and autoclaved at 15 Psi, 121 degree Celsius for 30 min on liquid cycle. Store at room temperature and protected from direct light..
20 Plates $50.00
LB-723 YPDA Medium
1% Yeast extract, 2% Peptone, 2% Dextrose, 0.02% Adenine. All ingredients dissolved in 18.2 megohms-cm ± 1 water and autoclaved at 15 Psi, 121 degree Celsius for 30 min on liquid cycle. Store at room temperature and protected from direct light..
500ml $30.00
1L $45.00

Yeast Transformation Media

Catalog # Product Name Pricing
BP-261 1X TE-LiAc
10 mM Tris HCl (pH 8.0), 1 mM EDTA, 0.1 M Lithium acetate. Prepared in 18.2 megohms water and filtered through 1 micron filter. Store at 2-8 degree Celsius.
100ml $40.00
BP-267 Beta-ME-EDTA Solution
90 mM Beta-Mercapto Ethanol and 40 mM EDTA. Prepared in 18.2 megohms water and filtered through 1 micron filter. Store at 2-8 degree Celsius.
250ml $40.00
BP-269 High Phosphate Buffer (5X HPB)
2.5 M NaCl, 0.9 M Na2HPO4, 25 mM Na2 EDTA (pH 7.0). Prepared in 18.2 megohms water and filtered through 1 micron filter. Store at 2-8 degree Celsius.
250ml $40.00
BP-256 Lisorb
100 mM Lithium acetate, 50 mM Tris-HCl (pH 8.0), 1 mM EDTA, and 1 mM Sorbitol. Prepared in 18.2 megohms water and filtered through 1 micron filter. Store at 2-8 degree Celsius.
250ml $40.00
BP-265 PEG 3350 (50%)
100ml $40.00
BP-257 Peg-Lioac Solution
10 mM Tris-HCl (pH 8.0), 1 mM EDTA, 100 mM Lithium acetate, and 40% PEG 3350. Prepared in 18.2 megohms water and filtered through 1 micron filter. Store at 2-8 degree Celsius.
250ml $40.00
BP-259 PEG-LiOAc Solution (10X)
50% PEG 4000, 1 M Tris HCl (pH 8.0), 1 M Lithium acetate (pH 7.5), 0.2 M EDTA. Prepared in 18.2 megohms water and filtered through 1 micron filter. Store at 2-8 degree Celsius.
100ml $40.00
BP-263 PEG-TE-LiAc
40% PEG 4000, 10 mM Tris HCl (pH 8.0), 1 mM EDTA, 0.1 M Lithium acetate. Prepared in 18.2 megohms water and filtered through 1 micron filter. Store at 2-8 degree Celsius.
100ml $40.00

Yeast Lysis Buffer

Catalog # Product Name Pricing
BP-271 Yeast Lysis Buffer
50 mM EDTA, 1% sarkosyl, 10 mM Tris-HCl, pH 8.0. Add 1 mg/ml Proteinase K before use. Prepared in 18.2 megohms water and filtered through 1 micron filter. Store at 2-8 degree Celsius.
250ml $45.00
BP-259B Yeast Lysis Buffer (for large scale prep)
0.5 M Tris-HCl (pH 8.0), 1% Sarkosyl, 0.2 M EDTA. Prepared in 18.2 megohms water and filtered through 1 micron filter. Store at 2-8 degree Celsius.
250ml $45.00
BP-253 Yeast Lysis Buffer (for mini prep)
50 mM Tris-HCl (pH 7.4), 25 mM EDTA (pH 8.0), 500 mM NaCl, 3 mM MgCl2, 3 mM Beta-Mercapto Ethanol, 0.1% Nonidet P-40, and 1.0% SDS. Prepared in 18.2 megohms water and filtered through 1 micron filter. Store at 2-8 degree Celsius.
250ml $45.00
BP-252 Yeast Lysis Solution for DNA Isolation
2.5 M LiCl, 50 mM Tris-HCl (pH, 8.0), 1% Triton X-100, and 62.5 mM EDTA. Prepared in 18.2 megohms water and filtered through 1 micron filter. Store at 2-8 degree Celsius.
250ml $45.00