REAGENTS FOR HISTOLOGY

For Histology we have commonly used fixatives(both formal-dehyde based and paraformaldehyde based), permeating solutions, antigenic enhancer, etc. If you have any specifications we will be glad to make it for you.

Permeabilization Buffers for IF, IHC, and ICC

Catalog # Product Name Pricing
BM-686 NP-40 permeating solution in PBS (10X)
1 % (v/v) NP-40 in 10X PBS. Prepared in 18.2 megohms water and filtered through 1-micron filter. stored at RT.
250ml $21.00
500ml $37.00
BM-687 NP-40 permeating solution in TBS (10X)
1 % (v/v) NP-40 in 10X TBS. Prepared in 18.2 megohms water and filtered through 1-micron filter. store at RT.
250ml $21.00
500ml $37.00
IBB-526 PBS with Digitonin
10 mM Sodium phosphate dibasic, 2 mM potassium phosphate monobasic, 2.7 mM KCl, 137 mM NaCl, and 0.1% digitonin, pH 7.4 ± 0.2. Prepared in 18 megohms water and was filtered using 0.22 micron filter. Store at room temperature. .
1ml $20.00
5x1ml $55.00
IBB-528 PBS with Digitonin (5 X)
50 mM Sodium phosphate dibasic, 10 mM potassium phosphate monobasic, 13.5 mM KCl, 685 mM NaCl, and 0.5% digitonin, pH 7.4 ± 0.2. Prepared in 18 megohms water filtered through 0.22 micron filter. Store at room temperature.
1ml $35.00
5 x 1ml $45.00
IBB-532 PBS with NP-40
10 mM Sodium phosphate dibasic, 2 mM potassium phosphate monobasic, 2.7 mM KCl, 137 mM NaCl, and 0.1% NP-40, pH 7.4 ± 0.2. Prepared in 18 megohms water and was filtered using 0.22 micron filter. Store at room temperature.
250ml $20.00
500ml $30.00
IBB-534 PBS with NP-40 (10 X)
100 mM Sodium phosphate dibasic, 20 mM potassium phosphate monobasic, 27 mM KCl, 1.37 M NaCl, and 1% NP-40, pH 7.4 ± 0.2. Prepared in 18 megohms water and was filtered using 0.22 micron filter. Store at room temperature.
250ml $30.00
500ml $45.00
IBB-536 PBS with Saponin
10 mM Sodium phosphate dibasic, 2 mM potassium phosphate monobasic, 2.7 mM KCl, 137 mM NaCl, and 0.1% saponin, pH 7.4 ± 0.2. Prepared in 18 megohms water and was filtered using 0.22 micron filter. Store at room temperature.
50ml $20.00
100ml $30.00
IBB-541 PBS with Saponin (5 X)
50 mM Sodium phosphate dibasic, 10 mM potassium phosphate monobasic, 13.5 mM KCl, 685 mM NaCl, and 0.5% saponin, pH 7.4 ± 0.2. Prepared in 18 megohms water filtered through 0.22 micron filter. Store at room temperature.
50ml $35.00
100ml $45.00
BM-220T PBS with Triton (10X)
100 mM Sodium phosphate dibasic, 20 mM Potassium phosphate monobasic, 27 mM KCl,1.37M NaCl and 1% Triton X-100, pH 7.4 ± 0.15 of 1X buffer.  Prepared in 18.2 megohms water and filtered through 1-micron filter. Store at RT.
250ml $21.00
500ml $37.00
IBB-543 PBS with Triton X-100
10 mM Sodium phosphate dibasic, 2 mM potassium phosphate monobasic, 2.7 mM KCl, 137 mM NaCl, and 0.1% Triton X-100, pH 7.4 ± 0.2. Prepared in 18 megohms water and was filtered using 0.22 micron filter. Store at room temperature.
250ml $20.00
500ml $30.00
IBB-170 PBST (10X, with 1% Tween-20, pH 7.4)
100 mM Sodium phosphate dibasic, 20 mM potassium phosphate monobasic, 27 mM KCl,1.37 M NaCl and 1% Tween-20, pH 7.4 ± 0.2. Prepared in 18.2 megohms water and autoclaved at 15 psi for 30 min on liquid cycle. The pH of the 1X should be 7.4 ± 0.2. To make 1X, dilute 10-fold with deionized water. Store at room temperature.
500ml $25.00
1L $40.00
2L $70.00
4L $125.00
BM-688 Saponine Permeating Solution in PBS (5X)
0.5 % Saponine w/v in 5X PBS (50 mM sodium phosphate dibasic, 10 mM potassium phosphate monobasic, 13.5 mM KCl and 0.685 M NaCl). Prepared in 18.2 megohms water and filtered through 1-micron filter.
125ml $30.00
250ml $45.00
BM-689 Saponine Permeating Solution in TBS (5X)
0.5 % Saponine w/v in 5X TBS (125 mM Tris-HCl, 13.5 mM KCl, 0.685 M NaCl), pH 7.4 ± 0.15. Prepared in 18.2 megohms water and filtered through 1-micron filter.
125ml $30.00
250ml $45.00
IBB-549 TBS-Standard Buffer with Digitonin
25 mM Tris-HCl, 137 mM NaCl, 2.7 mM KCl, and 0.1% Digitonin, pH 7.4 ± 0.2. Prepared in 18 megohms water and was filtered using 0.22 micron filter. Stored at room temperature.
1ml $20.00
5x1ml $30.00
IBB-554 TBS-Standard Buffer with Digitonin (5 X)
125 mM Tris-HCl, 685 mM NaCl, 13.5 mM KCl, and 0.5% Digitonin, pH 7.4 ± 0.2. Prepared in 18 megohms water and was filtered using 0.22 micron filter. Stored at room temperature.
1ml $35.00
5 x 1ml $45.00
IBB-556 TBS-Standard Buffer with NP-40
25 mM Tris-HCl, 137 mM NaCl, 2.7 mM KCl, and 0.1% NP-40, pH 7.4 ± 0.2. Prepared in 18 megohms water and was filtered using 0.22 micron filter. Stored at room temperature.
250ml $20.00
500ml $30.00
IBB-558 TBS-Standard Buffer with NP-40 (10 X)
250 mM Tris-HCl, 1.37 M NaCl, 27 mM KCl, and 1% NP-40, pH 7.4 ± 0.2. Prepared in 18 megohms water and was filtered using 0.22 micron filter. Stored at room temperature.
250ml $35.00
500ml $45.00
IBB-561 TBS-Standard Buffer with Saponin
25 mM Tris-HCl, 137 mM NaCl, 2.7 mM KCl, and 0.1% saponin, pH 7.4 ± 0.2. Prepared in 18 megohms water and was filtered using 0.22 micron filter. Stored at room temperature.
50ml $20.00
100ml $30.00
IBB-564 TBS-Standard with Saponin (5 X)
125 mM Tris-HCl, 685 mM NaCl, 13.5 mM KCl, and 0.5% saponin, pH 7.4 ± 0.2. Prepared in 18 megohms water and was filtered using 0.22 micron filter. Stored at room temperature.
50ml $35.00
100ml $45.00
IBB-567 TBS-Wash Buffer with Digitonin
50 mM Tris-HCl, 150 mM NaCl, and 0.1% digitonin, pH 7.4 ± 0.2 Prepared in 18 megohms water and was filtered using 0.22 mm filter. Store at room temperature.
1ml $20.00
5x1ml $30.00
IBB-569 TBS-Wash Buffer with Digitonin (5 X)
250 mM Tris-HCl, 750 mM NaCl, and 0.5% digitonin, pH 7.4 ± 0.2 Prepared in 18 megohms water and was filtered using 0.22 mm filter. Store at room temperature.
1ml $35.00
5 x 1ml $45.00
IBB-563 TBS-Wash Buffer with NP-40
50 mM Tris-HCl, 150 mM NaCl, and 0.1% NP-40, pH 7.4 ± 0.2 Prepared in 18 megohms water and was filtered using 0.22 mm filter. Store at room temperature.
250ml $20.00
500ml $30.00
IBB-566 TBS-Wash Buffer with NP-40 (10 X)
500 mM Tris-HCl, 1.50 M NaCl, and 1% NP-40, pH 7.4 ± 0.2 Prepared in 18 megohms water and was filtered using 0.22 micron filter. Store at room temperature.
250ml $35.00
500ml $45.00
IBB-571 TBS-Wash Buffer with Saponin
50 mM Tris-HCl, 150 mM NaCl, and 0.1% saponin, pH 7.4 ± 0.2 Prepared in 18 megohms water and was filtered using 0.22 mm filter. Store at room temperature.
50ml $20.00
100ml $30.00
IBB-573 TBS-Wash Buffer with Saponin (5 X)
250 mM Tris-HCl, 750 mM NaCl, and 0.5% saponin, pH 7.4 ± 0.2 Prepared in 18 megohms water and was filtered using 0.22 mm filter. Store at room temperature.
50ml $35.00
100ml $45.00
IBB-580 TBST-Standard (10X, with 1% Tween-20, pH 7.4)
250 mM Tris-HCl, 27 mM KCl, 1.37 M NaCl and 1% Tween-20, pH 7.4 ± 0.2. Prepared in 18.2 megohm water and autoclaved at 15 psi for 30 min on liquid cycle. Store at room temperature.
500ml $25.00
1L $40.00
2L $70.00
4L $125.00