LB-AGAR Spectinomycin (50 ug/ml)

LB-161



Composition 1% Tryptone, 0.5% yeast extract, 1% NaCl and 1.5% Agar. All the ingredients were dissolved in deionized water, pH was adjusted to 7.0 ± 0.2 and autoclaved at 15 psi, 121 degree Celsius for 30 min on liquid cycle. Following autoclaving the medium was cooled down to 50 to 55 degree Celsius before adding spectinomycin (50 ug/ml). Store at 2-8 degree Celsius.
MSDS Please call for MSDS Information
Storage 2-8 degree C
Grade Molecular biology grade
Appearance Brown/yellow
pH Not applicable.
Method Agar is mixed with LB-medium, followed by melting with autoclaving at15 PSI for 60 min, cooled to 52-50 degree C, mixed with or without antibiotics, poured onto petri-plates and let polymerized and dry under sterile aseptic condition.
Properties Boston Byproducts' LB Agar plates are manufactured under stringent possible condition with the supervision of BBP scientists and support persons.
Application This product is ideally suited for monitoring bacterial/microbial growth under nutrient-supplemented solidified platform.
Quality Control The process for this product includes clear solution of ingredient and operation under sterile aseptic condition.
Specification Boston BioProducts certifies that this product was inspected at all levels during its manufacturing, testing, and storage processes. The product conforms to all the specifications enlisted.

Similar Products from Boston BioProducts

BCA Protein Assay kit

BPA-500

Contains Reagent A & B. Method 1. Prepare samples containing 0.2-50 micro gm of protein in 10-20 ul. 2. Mix 50 parts of A and 1 part of B as working solution (WS). 3. Add 1 ml of WS to each samples and incubate at 60 Celsius for 30 min. 4. Cool the samples and read at 562 nm. 5. Color will be stable for at least 1 hr.

PBST (10X, with 1% Tween-20, pH 7.4)

IBB-170

100 mM Sodium phosphate dibasic, 20 mM potassium phosphate monobasic, 27 mM KCl,1.37 M NaCl and 1% Tween-20, pH 7.4 ± 0.2. Prepared in 18.2 megohms water and autoclaved at 15 psi for 30 min on liquid cycle. The pH of the 1X should be 7.4 ± 0.2. To make 1X, dilute 10-fold with deionized water. Store at room temperature.

Tris-Buffered Saline (TBS, 10X, pH 7.4)

BM-300

250 mM Tris, 27 mM KCl, and 1.37 M NaCl, pH 7.4 ± 0.2. Prepared in 18.2 megohms water and filtered through 1-micron filter. To make 1X, dilute 10-fold with DI water and the pH of the 1X should be 7.4 ± 0.2. Store at room temperature.